منابع مشابه
Ribonuclease selection for ribosome profiling
Ribosome profiling has emerged as a powerful method to assess global gene translation, but methodological and analytical challenges often lead to inconsistencies across labs and model organisms. A critical issue in ribosome profiling is nuclease treatment of ribosome-mRNA complexes, as it is important to ensure both stability of ribosomal particles and complete conversion of polysomes to monoso...
متن کاملRibosome Profiling: Principles and Variations
Ribosome profiling provides a snapshot of ribosome positions and density across the transcriptome at a sub-codon resolution. By sequencing the entire set of ribosome-protected mRNA fragments, this powerful approach has been successfully used to measure ribosome dynamics and reveal the hidden coding potential of transcriptome. Since its conceptual inception, ribosome profiling has evolved into a...
متن کاملRibosome dynamics during decoding
Elongation factors Tu (EF-Tu) and SelB are translational GTPases that deliver aminoacyl-tRNAs (aa-tRNAs) to the ribosome. In each canonical round of translation elongation, aa-tRNAs, assisted by EF-Tu, decode mRNA codons and insert the respective amino acid into the growing peptide chain. Stop codons usually lead to translation termination; however, in special cases UGA codons are recoded to se...
متن کاملThe awesome power of ribosome profiling.
Ribosome profiling has proved to be a fantastic innovation which has revealed so much about mRNA translation that had previously gone unrecognized. The RNA community owes Jonathan Weissman and Nick Ingolia a deep sense of gratitude for conceiving the approach in the first place, and for their detailed optimization of the methodology. The origins of the approach can be traced back to the discove...
متن کاملRibosome Profiling Enables Comprehensive Translation Analysis
Microarrays have the capacity to measure the expression levels of thousands of genes simultaneously1. Although microarrays have revolutionized mRNA analysis, they are only a proxy for the actual amount of protein synthesis occurring in cells. Multiple levels of transcriptional and post-transcriptional regulation contribute to the inherent challenges of proteomic analysis. A direct method for an...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Reviews Molecular Cell Biology
سال: 2016
ISSN: 1471-0072,1471-0080
DOI: 10.1038/nrm.2016.109